Open Data Service

Facilities

Biomedical Imaging Unit

Building: South Academic Block
Site: Southampton General Hospital

Equipment:

Imaging and Microscopy Centre

Building: Life Sciences
Site: Highfield Campus

Equipment:

Centre for Computed Tomography

Building: Eustice
Site: Highfield Campus

Equipment:

Equipment

Equipment: Hitachi H7000 Transmission Electron Microscope

125 kV, 600,000x magnification, plate camera, 1376x1032 camera, goniometer stage

Facility: Biomedical Imaging Unit

Features:

  • Transmission EM - TEM uses electron beam to image thin specimens/sections down to sub nanometer resolution …

Equipment: FEI Quanta 200 Scanning Electron Microscope

30 kV, 300,000x magnification, high and low vacuum detectors, environmental detector, backscatter detector, 3584x3094 camera, EDAX X-ray microanalysis

Facility: Biomedical Imaging Unit

Features:

  • Scanning EM - Surface image is created by scanning of fixed specimen with electron beam …
  • X-Ray Microanalysis - Analytical technique employing stimulated emission of X-rays from specimen …

Equipment: Leica SP5 Laser Scanning Confocal Microscope

inverted stand, 9 laser lines (405 to 633 nm), 4 spectral detectors, 1 transmitted light detector, AOBS, …

Facility: Biomedical Imaging Unit

Features:

Equipment: Olympus IX81 Live Cell Imaging System

inverted stand, x4 to x60 objectives, phase contrast, environmental chamber, fluorescence equipment, 1376 x 1032 camera, ...

Facility: Biomedical Imaging Unit

Features:

Equipment: Leica TCS SP2 multiphoton FCS

upright stand, multiphoton laser (710 to 990 nm), 8 laser lines (458 to 633 nm), 3 spectral detectors, 3 non-descanned detectors, 2 avalanche photo detectors, AOBS, …

Facility: Imaging and Microscopy Centre

Features:

  • Multiphoton Microscopy - The likelyhood of several photons co-exciting the same fluorescent molecule is only high within the focal plane …
  • Confocal Microscopy - A pinhole is used to cut out out-of-focus light in fluorescence images, thus generating thin optical sections …
  • Fluorescence Correlation Spectroscopy - FCS measures over time fluorescence fluctuations within the confocal volume …
  • Brightfield LM - Diffracted and undiffracted light creates an image on a bright background …
  • Fluorescence Microscopy - Light emitted from excited fluorescent molecules is used to from an image …
  • Time Lapse Microscopy - A series of microscopic images of the same specimen is acquired over time …

Equipment: Zeiss LSM 510 meta UV

upright stand, fixed stage, 8 laser lines (351 to 633 nm), 8 reflected light detectors, 1 transmitted light detector, monitor diode, ratiometric channels, …

Facility: Imaging and Microscopy Centre

Features:

Equipment: Zeiss Axiovert 200

inverted stand, x4 to x63 objectives, phase contrast, DIC, Halide lamp, 4164 x 3120 colour camera, …

Facility: Imaging and Microscopy Centre

Features:

  • Brightfield LM - Diffracted and undiffracted light creates an image on a bright background …
  • Fluorescence Microscopy - Light emitted from excited fluorescent molecules is used to from an image …
  • Time Lapse Microscopy - A series of microscopic images of the same specimen is acquired over time …

Equipment: Zeiss Axioskop 2 FS mot

upright stand, fixed stage, x4 to x63 objectives, DIC, HBO lamp, 1388 x 1040 camera, Axopatch 200B, Axoclamp-2A, …

Facility: Imaging and Microscopy Centre

Features:

  • Brightfield LM - Diffracted and undiffracted light creates an image on a bright background …
  • Fluorescence Microscopy - Light emitted from excited fluorescent molecules is used to from an image …
  • Time Lapse Microscopy - A series of microscopic images of the same specimen is acquired over time …
  • Electrophysiology - Measurement of the electrical properties of biological specimens …

Equipment: Gatan XuM/Zeiss Evo MA25 (LaB6 SEM hosted)

4-12 kV operation, ~200nm resolution, phase enhanced contrast, 1340x1300 camera, samples <= 1-2mm

Facility: Centre for Computed Tomography

Features:

Equipment: 225kV/450kV hutch: Nikon/Metris custom design

20-225kV and 100-450kV sources, down to ~3μm resolution, panel and line detectors, 1x1x1.5m imaging volume

Facility: Centre for Computed Tomography

Features:

Concepts

Concept: Brightfield LM

Diffracted and undiffracted light creates an image on a bright background …

Broader concepts: Light Microscopy

Equipment with this feature:

Concept: Computed Tomography

Broader concepts: Imaging
Narrower concepts: Micro-Computed Tomography

Concept: Confocal Microscopy

A pinhole is used to cut out out-of-focus light in fluorescence images, thus generating thin optical sections …

Broader concepts: Light Microscopy

Equipment with this feature:

Concept: Electron Micorscopy

Broader concepts: Imaging
Narrower concepts: Transmission EM, Scanning EM, X-Ray Microanalysis

Concept: Electrophysiology

Measurement of the electrical properties of biological specimens …

Broader concepts: Electrophysiology

Equipment with this feature:

Concept: Electrophysiology

Broader concepts: [NULL]
Narrower concepts: Electrophysiology

Concept: Fluorescence Correlation Spectroscopy

FCS measures over time fluorescence fluctuations within the confocal volume …

Broader concepts: Light Microscopy

Equipment with this feature:

Concept: Fluorescence Microscopy

Light emitted from excited fluorescent molecules is used to from an image …

Broader concepts: Light Microscopy

Equipment with this feature:

Concept: Imaging

Narrower concepts: Electron Micorscopy, Light Microscopy, Computed Tomography

Concept: Light Microscopy

Broader concepts: Imaging
Narrower concepts: Brightfield LM, Fluorescence Microscopy, Confocal Microscopy, Multiphoton Microscopy, Fluorescence Correlation Spectroscopy, Time Lapse Microscopy, Low Magnification Microscopy

Concept: Low Magnification Microscopy

Relatively large specimen are imaged with high spatial resolution …

Broader concepts: Light Microscopy

Concept: Micro-Computed Tomography

X-rays are used to create high-resolution 3D images from optical sections through a specimen ...

Broader concepts: Computed Tomography

Equipment with this feature:

Concept: Multiphoton Microscopy

The likelyhood of several photons co-exciting the same fluorescent molecule is only high within the focal plane …

Broader concepts: Light Microscopy

Equipment with this feature:

Concept: Scanning EM

Surface image is created by scanning of fixed specimen with electron beam …

Broader concepts: Electron Micorscopy

Equipment with this feature:

Concept: Time Lapse Microscopy

A series of microscopic images of the same specimen is acquired over time …

Broader concepts: Light Microscopy

Equipment with this feature:

Concept: Transmission EM

TEM uses electron beam to image thin specimens/sections down to sub nanometer resolution …

Broader concepts: Electron Micorscopy

Equipment with this feature:

Concept: X-Ray Microanalysis

Analytical technique employing stimulated emission of X-rays from specimen …

Broader concepts: Electron Micorscopy

Equipment with this feature:

Concept: [NULL]

Narrower concepts: Electrophysiology

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